In female mammals, Bartholin's glands open into the:. How to Get Rid of a Bartholin Cyst: 5 At Home Treatments The Bartholin's (BAHR toe linz) glands are located on each side of the vaginal opening. These glands secrete fluid that helps lubricate the vagina. Sometimes the openings of these glands become Bartholin gland masses UpToDate Vulva Definition, Anatomy, & Function Britannica
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